Purification and Characterization of Human Hypoxanthine/Guanine Phosphoribosyltransferase

Human hypoxanthine-guanine phosphoribosyltransferase. Purification and characterization of mutant forms of the enzyme.

Erythrocyte hypoxanthine-guanine phosphoribosyltransferase has been highly purified from five unrelated patients with a deficiency of this enzyme. Affinity chromatography using either GMP-Sepharose or an immunoadsorbent was the most productive step in the purifications. The specific activity of the purified enzyme was unchanged for patients L. P. and G. S., and slightly decreased for patient R....

Nicotinate phosphoribosyltransferase of yeast. Purification and properties.

synthesis and characterization of some macrocyclic schiff bases

ماکروسیکلهای شیف باز از اهمیت زیادی در شیمی آلی و دارویی برخوردار می باشند. این ماکروسیکلها با دارابودن گروه های مناسب در مکانهای مناسب می توانند فلزاتی مثل مس، نیکل و ... را در حفره های خود به دام انداخته، کمپلکسهای پایدار تولید نمایند. در این پایان نامه ابتدا یک دی آلدئید آروماتیک از گلیسیرین تهیه می شود و در مرحله بعدی واکنش با دی آمینهای آروماتیک و یا آلیفاتیک در رقتهای بسیار زیاد منجر به ت...

PURIFICATION AND CHARACTERIZATION OF THE CLONED HUMAN GM-CSF GENE EXPRESSED IN ESCHERICHIA COLI

The human granulocyte-macrophage colony stimulation factor (hGM-CSF) gene was cloned in the pET 23a( +) expression vector under the control of strong bacteriophage T7 transcription and translation signals. The hGM-CSF gene was transferred into E. coli strainBL21 (DE3)pLysS andIPTG was used for induction of GM-CSF gene. Production of the target protein was obtained as revealed by ELISA and ...

Expression, Purification and Characterization of Human Recombinant Galectin 3 in Pichia pastoris

Background: Over the past century, the areas of genomics, proteomics and lipids have captured the attention of investigators worldwide. Carbohydrates, have recently received increased attention through the expanding field of glycobiology; probably because they are very complex and not encoded in the genome. Objectives: The purpose of this study was to express and purify recombinant human galec...